PE-Labeled Human MSP1D1 Protein, His Tag (Nanodisc) (Site-specific conjugation)

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Cat. No. / Size
Price
Qty
APO-HP1E3-25tests
$320.00
APO-HP1E3-200tests
$1792.00
ETA of in-stock products:2 business days

Product Details

  • Synonym

    MSP1D1

  • Source

    PE-Labeled Human MSP1D1 Protein, His Tag (APO-HP1E3) is produced via site-specific conjugation of PE to Human MSP1D1 Protein, His Tag under optimal conditions with a proprietary technology. Human MSP1D1 Protein, His Tag is expressed from E. coli cells. It contains AA Ser 1 - Gln 211 (Accession # P11836-1).

    Predicted N-terminus: Met

    Request for sequence

  • Molecular Characterization

    MSP1D1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 29.4 kDa.

    Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by an amphipathic scaffold coat that confers enhanced stability and a narrow particle size distribution.

    The nanodisc assembles from a mixture of full length membrane protein in detergent, phospholipid micelles and membrane scaffold protein upon removal of the detergent.

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-FACS

     MSP1D1 FACS

    5e5 of CD20-CAR-293 cells transfected with anti-CD20-scFv were stained with 100 μL of 1:20 dilution (5 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human MSP1D1 Protein, His Tag (Cat. No. APO-HP1E3) and negative control protein respectively. PE-SA was used as negative control (QC tested).

    Protocol

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Background

Membrane scaffold proteins (MSPs) are synthetic derivatives of apolipoprotein A-I, a major protein component of human high-density lipoprotein complexes. Membrane scaffold protein 1D1 (MSP1D1) is the most common one among the MSPs variants. MSP1D1 is a synthetic derivate of apolipoprotein A-I, which is the major protein element of human high-density lipoproteins. The amphipathic, synthetic protein has the ability to self-assemble in the presence of synthetic phospholipids into discoidal nanoparticles, so called nanodiscs.

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