Biotinylated Human Claudin-3 Full Length Protein, His,Flag Tag&Avitag™ (Detergent)

Synonym

C7orf1, CPE-R2, CPETR2, HRVP1, RVP1

Source

Biotinylated Human Claudin-3 Full Length Protein, His,Flag Tag&Avitag (CL3-H85D3) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Val 220 (Accession # NP_001297.1).

Predicted N-terminus: Met

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the N-terminus and a Flag tag at the C-terminus followed by an Avi tag (Avitag™). The protein has a calculated MW of 28.7 kDa. The protein migrates as 18-19 kDa and 27-28 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the HABA assay / binding ELISA.

Purity

>90% as determined by SDS-PAGE.

Formulation

This product is not suitable for cell based experiments due to cytotoxicity of detergent.
Detergent buffer is INDISPENSABLE to keep membrane protein soluble and active, under no circumstances should you remove detergent.
Detergent buffer is sold separately and not included in protein, and please contact us if you need the buffer.
If glycerol is not compatible with your application, remove glycerol just before the immediate experiment, and NEVER store glycerol-free protein solution.

Supplied as 0.2 μm filtered solution in 50 mM HEPES, 150 mM NaCl, Buffer B, CHS, pH7.5 with glycerol as protectant.

Contact us for customized product form or formulation.

Shipping

This product is supplied and shipped with dry ice, please inquire the shipping cost.

Storage

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. The product MUST be stored at -70°C or lower upon receipt;
2. -70°C for 3 months under sterile conditions.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process