Mouse CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (MALS verified)

Synonym

CD3E & CD3D, CD3 delta & CD3 epsilon

Source

Mouse CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (CDD-M5219) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 108 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P22646-1 (CD3E) & P04235-1 (CD3D)).

Predicted N-terminus: Asp 23 (CD3E) & Phe 22 (CD3D)

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

Mouse CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 15.1 kDa (CD3E) and 14.3 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D is fused with a flag tag at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 18-19 kDa and 25-32 kDa under reducing (R) condition, and 38-47 kDa under non-reducing (NR) condition (SDS-PAGE).

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process