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Product Details
Synonym
CD3E & CD3D, CD3 delta & CD3 epsilon
Source
Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (CDD-C82W6) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # Q95LI5-1 (CD3E) & Q95LI8-1 (CD3D)).
Predicted N-terminus: Gln 22 (CD3E) & Phe 22 (CD3D)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 17.8 kDa (CD3E) and 13.5 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag and followed by an Avi tag (Avitag™) and subunit CD3D contains no tag at the C-terminus. The reducing (R) heterodimer protein migrates as 21-23 kDa (CD3E) and 19-21 kDa (CD3D) when calibrated against Star Ribbon Pre-stained Protein Marker due to glyosylation.
The protein is designed as a heterodimer.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-C82W6) is more than 95% and the molecular weight of this protein is around 35-55 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Bispecific BCMA×CD3 T cell-engaging Antibody (Cat. No. CDA-H52Hb) at 1 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-C82W6) with a linear range of 5-39 ng/mL (QC tested).
Protocol
Bridging ELISA
Validated with Bispecific Antibody.
Immobilized Cynomolgus DLL3 Protein, His Tag (Cat. No. DL3-C52H3) at 1 μg/mL, add increasing concentrations of Tarlatamab and then add Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-C82W6) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 3.91 ng/mL (Routinely tested).
Bioactivity-SPR
Bispecific T-cell Engager (CD3 X BCMA) immobilized on CM5 Chip can bind Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-C82W6) with an affinity constant of 53.5 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-C82W6) on SA Biosensor, can bind Human CD ×BCMA scFV with an affinity constant of 1.84 nM as determined in BLI assay (ForteBio Octet Red9e) (Routinely tested).
Protocol
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