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Product Details
Synonym
TNFRSF17, CD269, BCM, BCMA
Source
Rat BCMA, His Tag (BCA-R52H3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Thr 49 (Accession # D3ZKQ8-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 7.6 kDa. The protein migrates as 10-15 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Rat BCMA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) at 5 μg/mL (100 μL/well) can bind Rat BCMA, His Tag (Cat. No. BCA-R52H3) with a linear range of 0.039-0.625 μg/mL (QC tested).
Protocol
Immobilized Rat BCMA, His Tag (Cat. No. BCA-R52H3) at 5 μg/mL (100 μL/well) can bind Anti-BCMA Neutralizing Antibody, Mouse IgG1 (Cat. No. BCA-M43) with a linear range of 0.2-5 ng/mL (Routinely tested).
Protocol
Immobilized Rat BCMA, His Tag (Cat. No. BCA-R52H3) at 5 μg/mL (100 μL/well) can bind Mouse BAFF, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. BAF-M5257) with a linear range of 0.2-10 ng/mL (Routinely tested).
Protocol
Immobilized Rat BCMA, His Tag (Cat. No. BCA-R52H3) at 5 μg/mL (100 μL/well) can bind Human APRIL, Fc Tag with a linear range of 0.2-5 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
B-cell maturation protein
- Category：
- Approved Drugs：
8 Details
- Drugs in Clinical Trials：
145 Details
- Highest Development Stage：
Approved
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