Human BAFF & APRIL Heterotrimer Protein, His Tag (MALS verified)

Synonym

BAFF & APRIL

Source

Human BAFF & APRIL Heterotrimer Protein, His Tag (BAL-H5243) is expressed from human 293 cells (HEK293). It contains AA Glu 140 - Leu 285 & Gln 111 - Leu 250 (Accession # Q9Y275-1 & O75888-1).

Predicted N-terminus: His

Molecular Characterization

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 50 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. The protein is a heterotrimer consisting of one BAFF and two APRIL.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH6.0 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

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