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Product Details
Source
Monoclonal Anti-Human CD28 Antibody, Mouse IgG1 is a monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply.
Application
Flow Cytometry (Evaluation of the expression of CD28 on Human cells).
Species
Mouse
Isotype
Mouse IgG1 | Mouse Kappa
Specificity
This product is a specific antibody specifically reacts with CD28 protein.
Reactivity
Human
Immunogen
Purified Human CD28 Protein
Conjugate
APC
Excitation Wavelength: 640 nm
Emission Wavelength: 661 nm
Recommended Dilution
1:50
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300 and 0.2% BSA, with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
For best performance, we strongly recommend following the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 24 months in lyophilized state;
- -70°C for 12 months after reconstitution;
- 2-8 °C for 12 months after reconstitution.
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Performance Data
Bioactivity-FACS
Flow cytometric analysis of Human peripheral blood lymphocytes respectively staining with APC-Labeled Monoclonal Anti-Human CD28 Antibody, Mouse IgG1 (Cat. No. CD8-AHFC1) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. APC signal was used to evaluate the binding activity (QC tested).
Protocol
Non-specificity of APC-Labeled Monoclonal Anti-Human CD28 Antibody, Mouse IgG1 (Cat. No. CD8-AHFC1) binding to CD3- cells present in human PBMC. Human PBMCs were simultaneously stained with FITC anti-human CD3 Antibody and APC-Labeled Monoclonal Anti-Human CD28 Antibody, Mouse IgG1 (2 μL of the antibody stock solution corresponds to labeling of 5e5 cells in a final volume of 100 µL), washed and then analyzed with FACS. FITC negative signals and APC positive signals were used to evaluate the non-specific binding activity to human CD3- cells (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
T-cell-specific surface glycoprotein CD28
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
31 Details
- Highest Development Stage：
Phase 2 Clinical
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